milliplex® map human adipocyte magnetic bead panel Search Results


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Secretion of angiogenesis-related molecules and growth factors by AT-MSCs. A Concentration of selected angiogenesis-related molecules and growth factors in the conditioned media collected from healthy and diabetic AT-MSC culture. The AT-MSCs were cultured in the following media: standard CM (Control), CM with 25 mM glucose (Glucose) or CM with 25 mM glucose and 1 µg/ml insulin (Glucose + Insulin), and subsequently the listed media were replaced with αMEM supplemented with 1% HSA (for 24 h). To assess the concentration of analyzed molecules, <t>MILLIPLEX</t> Multiplex Assay using Luminex technology was used. Results are presented as mean ± SD, n = 3 (biological replicates); two-way Anova with Tukey’s multiple comparisons test. B Analytes not detected in AT-MSC-derived conditioned media. Healthy – healthy donor; T2D – diabetic donor
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Secretion of angiogenesis-related molecules and growth factors by AT-MSCs. A Concentration of selected angiogenesis-related molecules and growth factors in the conditioned media collected from healthy and diabetic AT-MSC culture. The AT-MSCs were cultured in the following media: standard CM (Control), CM with 25 mM glucose (Glucose) or CM with 25 mM glucose and 1 µg/ml insulin (Glucose + Insulin), and subsequently the listed media were replaced with αMEM supplemented with 1% HSA (for 24 h). To assess the concentration of analyzed molecules, <t>MILLIPLEX</t> Multiplex Assay using Luminex technology was used. Results are presented as mean ± SD, n = 3 (biological replicates); two-way Anova with Tukey’s multiple comparisons test. B Analytes not detected in AT-MSC-derived conditioned media. Healthy – healthy donor; T2D – diabetic donor
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Secretion of angiogenesis-related molecules and growth factors by AT-MSCs. A Concentration of selected angiogenesis-related molecules and growth factors in the conditioned media collected from healthy and diabetic AT-MSC culture. The AT-MSCs were cultured in the following media: standard CM (Control), CM with 25 mM glucose (Glucose) or CM with 25 mM glucose and 1 µg/ml insulin (Glucose + Insulin), and subsequently the listed media were replaced with αMEM supplemented with 1% HSA (for 24 h). To assess the concentration of analyzed molecules, <t>MILLIPLEX</t> Multiplex Assay using Luminex technology was used. Results are presented as mean ± SD, n = 3 (biological replicates); two-way Anova with Tukey’s multiple comparisons test. B Analytes not detected in AT-MSC-derived conditioned media. Healthy – healthy donor; T2D – diabetic donor
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Secretion of angiogenesis-related molecules and growth factors by AT-MSCs. A Concentration of selected angiogenesis-related molecules and growth factors in the conditioned media collected from healthy and diabetic AT-MSC culture. The AT-MSCs were cultured in the following media: standard CM (Control), CM with 25 mM glucose (Glucose) or CM with 25 mM glucose and 1 µg/ml insulin (Glucose + Insulin), and subsequently the listed media were replaced with αMEM supplemented with 1% HSA (for 24 h). To assess the concentration of analyzed molecules, <t>MILLIPLEX</t> Multiplex Assay using Luminex technology was used. Results are presented as mean ± SD, n = 3 (biological replicates); two-way Anova with Tukey’s multiple comparisons test. B Analytes not detected in AT-MSC-derived conditioned media. Healthy – healthy donor; T2D – diabetic donor
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Merck KGaA milliplex map kit human cardiovascular diseases magnetic bead panel 2
Secretion of angiogenesis-related molecules and growth factors by AT-MSCs. A Concentration of selected angiogenesis-related molecules and growth factors in the conditioned media collected from healthy and diabetic AT-MSC culture. The AT-MSCs were cultured in the following media: standard CM (Control), CM with 25 mM glucose (Glucose) or CM with 25 mM glucose and 1 µg/ml insulin (Glucose + Insulin), and subsequently the listed media were replaced with αMEM supplemented with 1% HSA (for 24 h). To assess the concentration of analyzed molecules, <t>MILLIPLEX</t> Multiplex Assay using Luminex technology was used. Results are presented as mean ± SD, n = 3 (biological replicates); two-way Anova with Tukey’s multiple comparisons test. B Analytes not detected in AT-MSC-derived conditioned media. Healthy – healthy donor; T2D – diabetic donor
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Secretion of angiogenesis-related molecules and growth factors by AT-MSCs. A Concentration of selected angiogenesis-related molecules and growth factors in the conditioned media collected from healthy and diabetic AT-MSC culture. The AT-MSCs were cultured in the following media: standard CM (Control), CM with 25 mM glucose (Glucose) or CM with 25 mM glucose and 1 µg/ml insulin (Glucose + Insulin), and subsequently the listed media were replaced with αMEM supplemented with 1% HSA (for 24 h). To assess the concentration of analyzed molecules, <t>MILLIPLEX</t> Multiplex Assay using Luminex technology was used. Results are presented as mean ± SD, n = 3 (biological replicates); two-way Anova with Tukey’s multiple comparisons test. B Analytes not detected in AT-MSC-derived conditioned media. Healthy – healthy donor; T2D – diabetic donor
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Image Search Results


Secretion of angiogenesis-related molecules and growth factors by AT-MSCs. A Concentration of selected angiogenesis-related molecules and growth factors in the conditioned media collected from healthy and diabetic AT-MSC culture. The AT-MSCs were cultured in the following media: standard CM (Control), CM with 25 mM glucose (Glucose) or CM with 25 mM glucose and 1 µg/ml insulin (Glucose + Insulin), and subsequently the listed media were replaced with αMEM supplemented with 1% HSA (for 24 h). To assess the concentration of analyzed molecules, MILLIPLEX Multiplex Assay using Luminex technology was used. Results are presented as mean ± SD, n = 3 (biological replicates); two-way Anova with Tukey’s multiple comparisons test. B Analytes not detected in AT-MSC-derived conditioned media. Healthy – healthy donor; T2D – diabetic donor

Journal: Cardiovascular Diabetology

Article Title: Comparison of biological properties of human adipose tissue-derived mesenchymal stem/ stromal cells from healthy and diabetic donors: consequences for cell-based medicinal product development

doi: 10.1186/s12933-025-02943-x

Figure Lengend Snippet: Secretion of angiogenesis-related molecules and growth factors by AT-MSCs. A Concentration of selected angiogenesis-related molecules and growth factors in the conditioned media collected from healthy and diabetic AT-MSC culture. The AT-MSCs were cultured in the following media: standard CM (Control), CM with 25 mM glucose (Glucose) or CM with 25 mM glucose and 1 µg/ml insulin (Glucose + Insulin), and subsequently the listed media were replaced with αMEM supplemented with 1% HSA (for 24 h). To assess the concentration of analyzed molecules, MILLIPLEX Multiplex Assay using Luminex technology was used. Results are presented as mean ± SD, n = 3 (biological replicates); two-way Anova with Tukey’s multiple comparisons test. B Analytes not detected in AT-MSC-derived conditioned media. Healthy – healthy donor; T2D – diabetic donor

Article Snippet: MILLIPLEX Human Angiogenesis/ Growth Factor Magnetic Bead Panel (Merck) containing beads for the following analytes: angiopoietin-2, endothelin-1, EGF, HB-EGF, VEGF-A, VEGF-D, leptin, follistatin, IL-8, FGF-1, FGF-2, HGF, PLGF, BMP-9, G-CSF was used to analyze the concentrations of the listed analytes in the conditioned media collected from AT-MSC cultures.

Techniques: Concentration Assay, Cell Culture, Control, Multiplex Assay, Luminex, Derivative Assay